14C-most-probable-number method for enumeration of active heterotrophic microorganisms in natural waters
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چکیده
منابع مشابه
The Most Probable Number Method and Its Uses in Enumeration, Qualification, and Validation
"Microbiology Topics" discusses various topics in microbiology of practical use in validation and com pliance. We intend this column to be a useful resource for daily work applications. Reader comments, questions, and suggestions are needed to help us fulfill our objective for this col umn. Please send your comments and suggestions to column coordinator Scott Sutton at scott.sutton@ microbiol...
متن کاملMost probable number methodology for quantifying dilute concentrations and fluxes of Salmonella in surface waters.
AIMS To better understand and manage the fate and transport of Salmonella in agricultural watersheds, we developed a culture-based, five tube-four dilution most probable number (MPN) method for enumerating dilute densities of Salmonella in environmental waters. METHODS AND RESULTS The MPN method was a combination of a filtration technique for large sample volumes of environmental water, stand...
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The large and diverse group of microscopic foodborne yeasts and moulds includes several hundred species. Both yeasts and moulds cause various degrees of deterioration and decomposition of food. They can invade and grow on virtually any type of food at any time, e.g. they invade field crops such as small grains, nuts, beans, tomatoes, and apples both in the field before harvesting and during sto...
متن کاملA most-probable-number assay for enumeration of infectious Cryptosporidium parvum oocysts.
Cryptosporidium is globally established as a contaminant of drinking and recreational waters. A previously described cell culture infectivity assay capable of detecting infectious oocysts was adapted to quantify viable oocysts through sporozoite invasion and clustering of foci. Eight experiments were performed by using oocysts less than 4 months of age to inoculate host HCT-8 cell monolayers. O...
متن کاملFactors influencing detection and enumeration of Pseudomonas aeruginosa by most-probable-number and membrane filtration techniques.
Most-probable-number (MPN) and membrane filtration (mF) techniques were evaluated with respect to selectivity, sensitivity, and efficiency in recovering Pseudomonas aeruginosa strains in hospital fluids and extramural water environments. Known numbers of cells of a naturally occurring strain of P. aeruginosa maintained in distilled water or cells subcultured on Standard Methods agar were added ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1979
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.38.4.644-649.1979